Analytical
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WuXi TIDES boasts a dedicated team of scientists with extensive experience and expertise in peptide analysis across all development phases. Equipped with state-of-the-art instruments and co-located with the API process development team, our peptide analytical service streamlines your peptide development process with a customized, flexible, and integrated analytical solution.
Our Services
- Method development and validation
- Stability Study: forced degradation study, accelerated and long-term stability study under R&D and GMP environment
- Purity and Assay: residual solvents, moisture, cation content, anion content, aggregation content, elemental impurities
- Microbiology Safety: endotoxin for limit and quantitation, bioburden for microbial limit test, microbe ID and strain typing
- Amino Acid Analysis and Enantiomeric Purity: acid hydrolysis and derivatization followed by LC-MS analysis to accurately evaluate the amino acid ratio and D-isomer impurity amount in peptide sequence
- Identity by Peptide Mapping: enzymatic digestion followed by LC-MS analysis for characterization and confirmation of peptide API
- Identity by Molecular Weight and Sequencing: 2D UPLC coupled with Q-TOF and MALDI-TOF/TOF HRMS for accurate MW and sequence determination
Peptide Enantiomeric Purity Test
Determining the enantiomeric purity of peptides accurately can be challenging. Peptides with different amino acid isomers share the same physicochemical properties, making them difficult to analyze intact. Free amino acids do not retain well for HPLC-UV analysis, and peptides containing multiple amino acids can interfere with each other during analysis. Despite these challenges, we have the capability to analyze the enantiomeric purity of all 19 isomeric natural amino acids (Asparagine and Aspartic acid, Glutamine and Glutamic acid are reported together), as well as Ornithine.
Chromatographic Techniques
- High Performance Liquid Chromatography (HPLC)
- High Performance Liquid Chromatography coupled with Mass Spectrometry (HPLC-MS)
- Ultra-High Performance Liquid Chromatography (UHPLC - available detectors: UV, CAD, MALS)
- Ultra-High Performance Liquid Chromatography coupled with Mass Spectrometry (UHPLC-MS)
- 2D Ultra-High Performance Liquide Chromatography (2D-UPLC)
- Gas Chromatography (GC)
- Ion Chromatography (IC)
- Thin Layer Chromatography (TLC)
Mass Spectrometry Techniques
- Electrospray Mass Spectrometry (ESI-MS Orbitrap & Q-TOF)
- Matrix Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-TOF/TOF)
- Tandem Mass Spectrometry Sequencing (Q-TOF, Orbitrap & MALDI-MS/MS)
- 2D Ultra-High Performance Liquide Chromatography coupled with HRMS (Q-TOF)
- Mass Spectrometry purity and impurity quantitation (Q-TOF)
- Gas Chromatography Coupled with Mass Spectrometer (GC-MS)
Spectroscopic Techniques
- Infrared Spectroscopy (IR)
- UV/VIS Spectroscopy
- Tm (UV melting curve)
- Circular Dichroism Spectroscopy (CD)
- Nuclear Magnetic Resonance Spectroscopy (1H, 13C, 31P & 19F - NMR)
Other Techniques
- Amino Acid Analysis (AAA)
- Elemental Analysis (CHN)
- Enantiomeric Purity
- Polarimetry
- Refractometry
- Titration
- Turbidimetry
- Osmolality
- Total Organic Carbon (TOC)
- Total Viable Aerobic Counts
- Endotoxins (LAL-test)
- Heavy Metals
- Sulfated Ash
- Residue on Ignition
- Polydispersity
