Peptide Discovery Synthesis
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Accelerate Peptide Synthesis with Our Strong Chemistry Capability
WuXi TIDES peptide discovery platform provides library synthesis and cusom synthesis to address your need and offer numerous options for discovery scales, purity levels, modifications, and salt forms.
In 2023, we provided custom peptide services for 400+ customers globally and delivered 30,000+ peptides from mg to kg scale.
We support the following peptide modalities, including but not limited to:
- Long Linear Peptide
- Cyclic Peptide
- Modified Peptide
- PEGylated Peptide
- Peptidomimetic
Service Highlights
Rich Experience
linear peptide up to 200 AAs, macrocyclic peptide, modified peptide and peptide based complex conjugates
Broad Scale
scale from mg to kg, purity up to 99.9%
High Success
Rate
over 98.5% on time delivery
Comprehensive Analysis Support
default purity/MS profile and additional services
High-Throughput Peptide Synthesis
rich experience in peptide structure-activity-relationship (SAR) study, range from 25 µmol to 1.0 mmol
Quick Turnaround
Time
2 weeks turnaround time for most peptides under 30 AA at mg scale
Deliverables
Form
- Lyophilized powder
- Stock solution
Certificate of Analysis
- HPLC/UPLC and LCMS
- ID, barcode of vials
- Sequence/structure
- Quantity, purity
- Storage condition, etc.
Packaging and Logistics
- Nitrogen packaging available
- Ambient logistics worldwide
- With icebag
- Dry ice logistics with optional thermal control
Note: Additional Services (TFA removal, analytical HPLC, etc.) are available at additional cost
Library Synthesis
Estimated output based on routine peptide sequence:
| Item | <10 AAs | 10-20 AAs | 20-30 AAs |
|---|---|---|---|
| Number of Peptides | 48 peptides in parallel synthesis | ||
| Delivery Time | <8 days | <12 days | <20 days |
|
▸
Peptide Companion Prediction-assisted assessment can be applied prior to automated synthesis.
▸ Insoluble (and/or expensive) AAs (building blocks) are not recommended for automated synthesis. ▸ The microwave-assisted synthesizer excels in facilitating difficult peptide synthesis. ▸ The combination of automated and manual synthesis is employed to expedite the process of long peptide synthesis and enhance the success rate. |
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Note:
1. Turnaround time may be extended for orders exceeding 48 peptides, sequences with high N-Me content, challenging modifications, or salt exchange requirements.
2. The turnaround time encompasses all business days from order confirmation to completion, excluding shipping duration.
3. Additional services, such as TFA removal and analytical HPLC, are available at an extra cost.
Custom Synthesis
Linear Peptide Synthesis
Approach:
- Fmoc Solid-Phase Peptide Synthesis (SPPS)
- Fragment Coupling
- Ligation
Speed:
- 2-30 AA, 5-13 days
- 31-60 AA, 13-30 days
- 60-130 AA, conjugation/ligation, case by case
Cyclic Peptide Synthesis
Cyclization is a prevalent strategy employed to enhance the pharmacological properties of a peptide from its linear precursor. These enhancements may include improved affinity and specificity, proteolytic stability, and/or solubility.
Our team possesses a wealth of experience in cyclic peptide synthesis, having successfully delivered over 100,000 cyclic peptides.
We utilize a variety of cyclization strategies such as single/double/triple disulfide, click cyclization, lactone, lactam, and RCM. The possible cyclization positions include head-to-tail, head-to-sidechain, sidechain-to-sidechain, and sidechain-to-tail.
Head to Tail
Head to Sidechain
Sidechain to Sidechain
Sidechain to Tail
Experience in:
- Disulfide Peptide (single/double/ triple disulfide)
- Thioether Cyclization
- Lactam Cyclization
- Lactone Cyclization
- Click Peptide
- RCM Cyclization
- Bicyclic Peptide
Bicyclo-disulfide Bridge
(Three pairs of S-S bond formation achieved as well)
RCM Monocyclic Peptide
Bicyclic Peptide
Click Peptide
Thioether Cyclization
Modified Peptide Synthesis
We offer a comprehensive range of specialized modifications to meet your specific requirements. These include N-terminal modifications, C-terminal modifications, dye/fluorescent labeling, biotin-labeling, PEGylation, post-translational modifications, DOTA/NOTA labeling, and isotope labeling.
Beyond sidechain and backbone modifications, we are also capable of incorporating unnatural amino acids into the sequences. For more information on our catalog of unnatural amino acid products, please visit here.
Modified Peptide Synthesis
- H (free amine)
- Acetylation
- Fmoc
- CBZ
- Bz
- Pyr
- Biotinylation
- Lipid tail
- Dye and Fluorescent labeling
- -OH (free)
- Ester (-Ome)
- -NH2
- -NH-Me
- Active ester (NHS; TFP; PFP)
- Dye and Fluorescent (by conjugation)
- Biotin；
- FITC；
- 5-FAM (5,6-FAM)；
- Dansyl-
- TAMTA-
- MCA-
- Rhodamine
- Acetylation (Lys)
- Methylation (Arg)
- Phosphorylation (Ser, Thr, Tyr)
- Lipidation
- Glycosylation
- SUMOylation
- Ubiquitionation
- 1H – D
- 12C – 13C
- 14N – 15N
- 13C, 15N – Lys
- 13C, 15N – Arg
- 13C, 15N – Ala
- 13C, 15N – Ile
- 13C, 15N – Leu
- 13C, 15N – Pro
- 13C, 15N – Phe
- 13C, 15N – Val
- N-linked glycopeptide synthesis
- O-linked glycopeptide synthesis
- C-linked glycopeptide synthesis
- S-linked glycopeptide synthesis
Peptide Library Synthesis
Automatic Peptide Synthesizer
For routine peptide synthesis, automatic synthesizers can accelerate Solid Phase Peptide Synthesis portion. Our synthesizers include CSBio, CEM Liberty Blue, and Biotage Syro II. With these advanced synthesizers, as well as a variety of prep-HPLC equipment installed in house, your requested compounds can be ready within as fast as 5 days.
CEM Liberty Blue
- As fast as 5 min/position
- Microwave synthesizer
- 12-channel
- Sequential synthesis
- 0.1-0.2 mmol
Biotage Syro II
- High throughput synthesizer
- 24/48/96 channel
- Parallel synthesis
- Suitable for SAR screening
- 25 umol
CSBio Synthesizer
- 3-channel
- Parallel synthesis
- 0.2-0.5 mmol
Related Resources
WuXi TIDES Brochure: CRDMO Platform for Oligonucleotide, Peptide and Complex Conjugate
Peptide CRDMO Chemistry Platform
